Genome editing and its role in vaccine, diagnosis, and therapeutic advancement
Document Type
Article (peer-reviewed)
Publication Date
6-1-2024
Abstract
Genome editing involves precise modification of specific nucleotides in the genome using nucleases like CRISPR/Cas, ZFN, or TALEN, leading to increased efficiency of homologous recombination (HR) for gene editing, and it can result in gene disruption events via non-homologous end joining (NHEJ) or homology-driven repair (HDR). Genome editing, particularly CRISPR-Cas9, revolutionizes vaccine development by enabling precise modifications of pathogen genomes, leading to enhanced vaccine efficacy and safety. It allows for tailored antigen optimization, improved vector design, and deeper insights into host genes' impact on vaccine responses, ultimately enhancing vaccine development and manufacturing processes. This review highlights different types of genome editing methods, their associated risks, approaches to overcome the shortcomings, and the diverse roles of genome editing.
Recommended Citation
Bisht, Deepanker, Sagar Salave, Nimeet Desai, Purnima Gogoi, Dhwani Rana, Prachurya Biswal, Gautami Sarma, Derajram Benival, Nagavendra Kommineni, and Dhruv Desai. 2024. "Genome editing and its role in vaccine, diagnosis, and therapeutic advancement," International Journal of Biological Macromolecules 269: Article 131802.
DOI
10.1016/j.ijbiomac.2024.131802
Language
English
https://doi.org/10.1016/j.ijbiomac.2024.131802