Title

Enhanced chemiluminescence (ECL) for routine immunoblotting. An inexpensive alternative to commercially available kits

Document Type

Article (peer-reviewed)

Publication Date

2011

Abstract

Immunoblotting is an analytical technique used by many laboratories to study protein expression. It involves electrophoretic separation of proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ), immobilization of these proteins onto a membrane of either nitrocellulose or polyvinylidene difluoride, incubation of the membrane in a monoclonal or polyclonal antibody and detection by a standard method such as enhanced chemiluminescence (ECL). To achieve this, most laboratories opt to use commercially-available chemiluminescence kits which are acceptable but relatively expensive. In this technical report, we show that a self-prepared chemiluminescence reagent is superior to a commercially obtained kit in terms of sensitivity, duration of signal, ease-of-use and shelf-life but at a fraction of the cost of a kit.

DOI

10.4161/spmg.1.2.16606

Language

English

Project

The Biology of Blood–Testis Barrier Dynamics

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