Enhanced chemiluminescence (ECL) for routine immunoblotting. An inexpensive alternative to commercially available kits
Document Type
Article (peer-reviewed)
Publication Date
2011
Abstract
Immunoblotting is an analytical technique used by many laboratories to study protein expression. It involves electrophoretic separation of proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ), immobilization of these proteins onto a membrane of either nitrocellulose or polyvinylidene difluoride, incubation of the membrane in a monoclonal or polyclonal antibody and detection by a standard method such as enhanced chemiluminescence (ECL). To achieve this, most laboratories opt to use commercially-available chemiluminescence kits which are acceptable but relatively expensive. In this technical report, we show that a self-prepared chemiluminescence reagent is superior to a commercially obtained kit in terms of sensitivity, duration of signal, ease-of-use and shelf-life but at a fraction of the cost of a kit.
Recommended Citation
Mruk, Dolores D. and C. Yan Cheng. 2011. "Enhanced chemiluminescence (ECL) for routine immunoblotting. An inexpensive alternative to commercially available kits," Spermatogenesis 1(2): 121–122.
DOI
10.4161/spmg.1.2.16606
Language
English
Project
The Biology of Blood–Testis Barrier Dynamics