Identification of personal lubricants that can cause rectal epithelial cell damage and enhance HIV type 1 replication in vitro
Document Type
Article (peer-reviewed)
Publication Date
2011
Abstract
Over-the-counter personal lubricants are used frequently during vaginal and anal intercourse, but they have not been extensively tested for biological effects that might influence HIV transmission. We evaluated the in vitro toxicity anti-HIV-1 activity and osmolality of popular lubricants. A total of 41 lubricants were examined and compared to Gynol II and Carraguard as positive and negative controls for toxicity, respectively. Cytotoxicity was assessed using the XTT assay. The MAGI assay with R5 and X4 HIV-1 laboratory strains was used to evaluate antiviral activity. The effect of the lubricants on differentiated Caco-2 cell monolayers (transepithelial electrical resistance, TEER) was also measured. None of the lubricants tested showed significant activity against HIV-1. Surprisingly, four of them, Astroglide Liquid, Astroglide Warming Liquid, Astroglide Glycerin & Paraben-Free Liquid, and Astroglide Silken Secret, significantly enhanced HIV-1 replication (p
Recommended Citation
Begay, Othell, Ninochka Jean-Pierre, Ciby J. Abraham, Anne Chudolij, Samantha Seidor, Aixa Rodriguez, Brian E. Ford, Marcus Henderson, David Katz, Thomas Zydowsky, Melissa Robbiani, and José Fernández-Romero. 2011. "Identification of personal lubricants that can cause rectal epithelial cell damage and enhance HIV-1 replication in vitro," AIDS Research and Human Retroviruses 27(9): 1019–1024.
DOI
10.1089/aid.2010.0252
Language
English
Project
Safety of Sexual Lubricants