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The Multi-Sectoral Approaches to Preventing Sexual Assault and Domestic Violence Symposium took place May 6–7, 2009, in Gauteng, South Africa. The symposium aimed to: increase awareness of sexual assault and domestic violence and its relationship to HIV and AIDS among decision-makers; promote evidence-based programming for practitioners; advocate for increased preventative measures and service delivery around sexual and gender-based violence (SGBV) in the public and private sectors; facilitate networking, strengthen and develop partnerships, and create opportunities for ongoing collaboration between key stakeholders; and inspire a coordinated plan of action for prevention that supports the government’s 365-day plan. South Africa has some of the highest SGBV figures in the world. This is a violation of the human rights of women and girls and also a major cause of mortality and morbidity. In addition, SGBV is a key driver of the HIV epidemic for women. Existing male norms also serve to fuel the SBGV and HIV epidemics. Key findings of the symposium and recommendations for addressing risks are included in this symposium report.