An in vitro system to study sertoli cell blood-testis barrier dynamics
The use of an in vitro system based on primary cultures of Sertoli cells isolated from rat testes has greatly facilitated the study of the blood-testis barrier in recent years. Herein, we summarize the detailed procedures on the isolation of undifferentiated Sertoli cells from 20-day-old rat testes, the culture of these cells as a monolayer on Matrigel-coated bicameral units, the characterization of these cultured cells, and the use of the Sertoli cell epithelium for monitoring the integrity of the Sertoli cell blood-testis barrier. This information is based on the routine use of this system in our laboratory to study the Sertoli cell blood-testis barrier in the past two decades, which should be helpful for investigators in the field.
Mruk, Dolores D. and C. Yan Cheng. 2011. "An in vitro system to study Sertoli cell blood-testis barrier dynamics," Methods in Molecular Biology 763: 237–252.
The Biology of Blood–Testis Barrier Dynamics