Inhibition of sperm capacitation and fertilizing capacity by adjudin is mediated by chloride and its channels in humans
Study Question: Does adjudin disrupt chloride ion (Cl^–) ion transport function in human sperm and impede sperm capacitation and fertilizing ability in vitro? Summary Answer: In this study the results indicate that adjudin is a potent blocker of Cl^– channels: disrupting Cl^– ion transport function results in a decline in sperm capacitation and fertilizing ability in humans in vitro. What is Known Already: Although our previous studies have demonstrated that adjudin exerts its effect by disrupting sertoli–germ cell adhesion junctions, most notably apical ectoplasmic specialization by targeting testin and actin filament bundles that disrupts the actin-based cytoskeleton in sertoli cells, it remains unclear whether adjudin impedes Cl^– ion transport function in the human sperm. Study Design, Size, and Duration: Semen samples were obtained from 45 fertile men (aged 25–32). Spermatozoa were isolated from the semen in the human tube fluid (HTF) medium by centrifugation through a discontinuous Percoll gradient, and incubated with adjudin at 10 nM–10 µM and/or other reagents under capacitating conditions for 0–5 h. Participants/Materials, Setting, Methods: We evaluated the effect of adjudin and different reagents on sperm functions with which they were incubated at 37°C. Sperm motility and hyperactivation were analyzed by a computer-assisted sperm analysis (CASA) system. Sperm capacitation and the acrosome reaction were assessed by chlortetracycline fluorescence staining. Sperm fertilizing ability was evaluated by sperm penetration of zona-free hamster egg assay, and cellular cAMP levels in spermatozoa were quantified by the EIA kit. The proteins tyrosine, serine and threonine phosphorylation in the presence or absence of adjudin were analyzed by means of a immunodetection of spermatozoa, especially, compared the effect of adjudin on sperm hyperactivation and capacitation in the complete HTF medium with the Cl^–-deficient HTF medium as well as the various Cl^– channel blockers. Main Results and the Role of Chance: Adjudin significantly inhibited sperm hyperactivation but not sperm motility. Adjudin-induced inhibition of sperm capacitation was reversible, and it was found to block the rhuZP_3β- and progesterone-induced acrosome reaction in a dose-dependent manner. Adjudin also blocked sperm penetration of zona-free hamster eggs, and significantly inhibited both forskolin-activated transmembrane adenylyl cyclase and soluble adenylyl cyclase activities leading to a significant decline in the cellular cAMP levels in human spermatozoa. Adjudin failed to reduce sperm protein tyrosine phosphorylation but it did prevent sperm serine and threonine protein phosphorylation. Interestingly, adjudin was found to exert its inhibitory effects on sperm capacitation and capacitation-associated events only in the complete Cl^–-HTF medium but not Cl^–-deficient medium, illustrating the likely involvement of Cl^–. Adjudin inhibits the fertility capacity of human sperm is mediated by disrupting chloride ion and its transport function. Limitations, Reasons for Caution: This study has examined the effect of adjudin only on human sperm capacitation and fertilizing ability in vitro and thus has some limitations. Further investigations in vivo are needed to confirm adjudin is a potent male contraceptive. Wider Implications of the Findings: Our studies demonstrated that adjudin inhibition of capacitation is reversible and its toxicity is low, opening the door for the examination of adjudin as a mediator of male fertility control. Adjudin may be a safe, efficient and reversible male antifertility agent and applicable to initial clinical trials of adjudin as a male antifertility agent in humans.
Li, Kun, Ya Ni, Yi He, Wen-Ying Chen, Jian-Xin Lu, C. Yan Cheng, Renshan Ge, and Qi-Xian Shi. 2013. "Inhibition of sperm capacitation and fertilizing capacity by adjudin is mediated by chloride and its channels in humans," Human Reproduction 28(1): 47–59.
Development of Adjudin as a Male Contraceptive